Methods in Microbiology, Volume 32 presents up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analyzing host -- pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology, and parasitology. This volume provides ready-to-use "recipes" that have been updated to give the latest cutting-edge protocols on emerging techniques as well as new approaches and insights into the tried and tested established methods included in the successful first edition.