Clamp Loader of Escherichia Coli Dna Polymerase III: Kinetics of the Atp-dependent Steps in the Sliding-clamp Loading Reaction

Abstract:

Sliding clamps tether DNA polymerases to DNA to increase the processivity of synthesis. The Escherichia coli " complex loads the ß sliding clamp onto DNA in an ATP-dependent reaction in which ATP binding and hydrolysis modulate the affinity of the " complex for ß and DNA. This dissertation covers research describing the kinetic mechanism of clamp loading by gamma complex.

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